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When you do bioassays, a 96-well plate is the ideal container. Its 96-well design makes it ideal for performing large-scale in-vitro experiments. The 96-well format facilitates toxicology and ADME screening because of its ability to accommodate large homogeneous batches of relevant cell types. Although many commercial suppliers offer plated cells, cryopreserved cells can also be seeded into plates.

When performing an experiment, you must first determine the number of cells. When a 96 Well Plates has a volume of 96 mL, you must multiply the number of cells by this volume. This calculation can be done by either multiplying the cell number by 100 or using a multi-channel pipette. When comparing two wells with the same cell line, remember that the cells in the larger well will be diluted to a much smaller volume than the one in the small well. The resulting cell density is expressed in millions per mL of culture.

The method of Gently Centrifugation has been demonstrated to be easy to perform and is ideal for experiments with a small number of cells. This method can be combined with other 96-well plate based assays to assess multiple endpoints of cell death. This allows the researchers to compare the number of cells in a given plate with different cell types. Moreover, the study can be conducted with very small numbers of cells and very short cultures.

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