Imaging: Needs Assessment

To identify the imaging needs of the different groups/users in the department

CURRENT USE

(more than one choice possible)

I routinely use:

Non-fluorescent imaging (Brightfield or Darkfield microscopy)

Fluorescence microscopy (GFP etc.) for fixed cells

Fluorescence microscopy (GFP etc.) for live cells

Single particle fluorescent imaging (SPFI)

Whole organism microscopy (e.g. embryo, root hair...)

Flow cytometry

Bioluminescence imaging

Other (please specify):

AVERAGE TIME SPENT

(more than one choice possible)

Microscopy: I spend 1-2 hours a week

Microscopy: I spend 3-6 hours a week

Microscopy: I have pale skin from living in the darkroom

Flow cytometry: I spend 1-2 hours a week

Flow cytometry: I spend 3-6 hours a week

Flow cytometry: The cytometer and I are <i>one</i>

Image data analysis: I spend 1-2 hours a week

Image data analysis: I spend 3-6 hours a week

Image data analysis: I do nothing but

MOST IMPORTANT PARAMETERS

(more than one choice possible)

The most important parameter(s) to me is (are):

Speed (I image fast processes)

Magnification (I image very small structures)

Signal strength (I am dealing with weak fluorescence)

Longevity (I keep my cells happy for a long time)

Large field of view (I image large structures)

Not sure

Other (please specify):

RECORDING LENGTH

(more than one choice possible)

my recordings usually last around

milliseconds

seconds

minutes

hours

days

(and/or) I use fixed cells (time is not an issue)

Not sure

BRIGHTFIELD MICROSCOPY

i.e. non-fluorescent microscopy (using a halogen light source)

(more than one choice possible)

I donÃ¢ÂÂt use brightfield at all

I use brightfield to find my cells/structures before fluorescent imaging

I use brightfield to show cell/structure outlines for the fluorescent imaging

I use brightfield for stained histology specimens

I use brightfield for time-lapse imaging

I use Phase Contrast (PhaCo)

I use DIC (= Nomarski)

Not sure

TECHNIQUES, INSTRUMENTS, PROGRAMS

more than one choice possible

Of the following, I (or my group) use:

DATA ANALYSIS

(more than one choice possible)

I usually use:

Particle tracking

Time-independent localisation (usually in fixed cells)

Time-dependent localisation (in live cells)

3D reconstruction

Co-localisation studies of two proteins

Co-localisation studies of three proteins

Co-localisation studies of four proteins

Not sure

Other (please specify):

PERSPECTIVE

(more than one choice possible)

In 3-5 years from now, I would most like to use

FLUORESCENT TAGS

(more than one choice possible)

I (my group) use one or several of the following fluorophores:

This poll was created on 2009-11-17 10:06:45 by philippe laissue